Gxene Biotech has been working on recombinant protein expression and antibody development, and has maintained high-quality requirement to produce clean and reproducible results for purification. You have access to customized protein purification services, including tag/tag-free recombinant proteins, purification and separation of natural proteins. Our laboratory has more than a dozen GE purification resins that enable simultaneous large-scale scanning protein purification and separation, saving you time and money.   
Natural Protein Purification Service:
 

Project Name	Protocol
Separation and purification of natural macromolecular proteins	* Separation and purification proteins using molecular sieves of appropriate pore size, combined with anion and cation adsorption columns and HLPC, identify and collect corresponding elution peaks.
Separation and purification of natural small molecular proteins	* Multi-layer separation and filtration combined with molecular sieve to remove macromolecular impurities, combined with macroporous resin or reverse resin to purify small molecular proteins, purified by HPLC and MS.
Separation and purification of mixed unknown protein samples	* Separation and purification were carried out by Gxene Biotech, and identified by mass spectrometry, activity identification, etc.

 

Recombinant Protein Purification Services:
 

Project Name	Protocol
Deproteinization, separation and purification of recombinant proteins	* Commercially purified resin, such as Ni column, GST-Beads, etc., and purified with 6xHis, GST, SUMO, MBP to obtain a fusion protein with a purity > 90%.
Tag free recombinant protein purification	* Antibody affinity purification; also can be selected similar to the natural protein purification method, depending on the project.
Inclusion body denaturation and renaturation purification	* According to the different properties of the protein, urea, guanidine hydrochloride, detergent, extreme pH, etc. can be selected to ensure the monomeric state's maximum denaturation.