Gxene Biotech considers technology to be its heart and has refined the antibody production platform over the years, supported by advanced antibody engineering technology.  Phosphorylation events are commonly probed by phosphorylation-specific antibodies. With many years of in-depth research in antibody phosphorylation, we can provide high-quality phosphorylated antibody production services, including synthesis of phosphorylated and non-phosphorylated peptides, conjugation to a carrier protein, immunization, serum production and affinity purification of the antibodies. Produce high-affinity antibodies that recognize this most widely studied post-translational modification, phosphorylation.  
Phosphorylated Antibody Production:
 
 
Service Procedure:
 

Steps	Specification	Deliverables
Antigen design and synthesis	* Clients provide (phosphopeptide, non-phosphopeptide), purity >90% * Clients provide target antigen sequence (polypeptide or protein sequence and Phosphorylation site) * We design and synthesize phosphorylated or non-phosphorylated antigen peptides (Optional)	(Antigen production by Gxene Biotech) * MS and HPLC reports (for both phosphopeptide and non-phosphopeptide). * 10mg phosphopeptide (>90% purity). * 2-5mg non-phosphopeptide (>90% purity).
Animal immunization	* Protocol 1: Immunization of 5 Balb/c mouse (using a unique mouse immunoadjuvant to produce high titer antiserum rapidly in 20 days). Detection of serum titer by ELISA. * Protocol 2: Immunization of 2-3 rabbits (using a unique rabbit immunoadjuvant to produce high titer antiserum rapidly in 28 days). Detection of serum titer by ELISA. (Other animals are available)	* 1-2 ml of pre-immune serum. * ELISA identification report.
Production of antibody phage display libraries	* Collecting B lymphocytes after immunization. * mRNA extraction & cDNA synthesis & phage vector construction & transformation & amplification. * Screening of antibody library with phosphorylated and non-phosphorylated antigens. * Amplification of positive clones & Construction of shuttle plasmids. * Small-scale and large-scale mammalian system expression (293, CHO). Antibody purification and identification.	* Recombinant expression plasmid. * 2-5 mg of purified recombinant antibody. * ELISA results (both for phosphopeptide and nonphosphopeptide). * Western Blot identification report (Optional).