Gxene Biotech is a professional provider of antibody and recombinant protein products and services, with years of pre-clinical research experience and production experience to provide upstream and downstream services for antigen and antibody production. Whether you have a clearly defined protocol or you just have an idea for an experiment, Gxene Biotech can tailor it to your needs.
Antibody Purification - Protein A / G affinity Purification:
Protein A is a cell wall protein isolated from S. aureus that binds to mammalian IgG via the Fc region. Protein A can be used to isolate and purify IgG and its subclasses and fragments. The Protein A agarose column is suitable for immunoprecipitation of murine IgG2a, IgG2b, IgA, human IgG1, IgG2 and IgG4.
Protein G is a cell wall protein isolated from G-type Streptococcus that binds to mammalian IgG via the Fc region. Protein G Agarose is more suitable for immunoprecipitation of murine IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit and sheep polyclonal antibodies, and human IgG1 , IgG2, IgG3 and IgG4.
Main Process:
Fig1 Protein A / G affinity Purification
Protein A/G Affinity Purification Packages:
--Customer provides antiserum, and we will purify antibodies (IgG, IgM, IgD, IgA and IgE) according to the customers' specific requirements
--Purification scale: μg ~g
--Quality Report: antibody concentration, SDS-PAGE analysis results
Antibody Purification - Ligand Affinity Purification:
Gxene Biotech can provide a ligand chromatography column according to the antibody or antigen supplied by the customer; as shown in Figure 2, beads are agarose gel column or magnetic beads (customized or recommended by us); linker can be added to reduce the steric effect between biomacromolecules.
Fig 2 ligand chromatography column
Applications of Affinity Chromatography:
--Purified biomacromolecules
--Concentration of dilute solution
--Storage of unstable proteins
--Remove residual contaminants from purified molecules
--Adsorption and purification of biomacromolecules without complementary ligands by immunosorbent
--Isolation of nucleic acids is an essential aspect of affinity chromatography applications