Gxene Biotech strives to offer high-quality antibody production to our customers. Mouse monoclonal antibody has many features such as targeting to a single epitope, high specificity, stable passage, large-scale manufacturing and extensive applications in basic research, medical diagnosis, tumor therapy. Backed by upgraded technology and extensive practical experience, our scientific team has developed mouse monoclonal antibodies with high sensitivity and specificity to bring a better outcome for your downstream services.
To give you more choice and save you time, Gxene Biotech is engaged in providing comprehensive services to meet your different needs. At present, Gxene Biotech can provide two mouse monoclonal antibody production protocols: cell fusion and phage display library construction technologies. Meanwhile, under the assistance with our well-established and powerful antibody engineering platform, antigen design, peptide synthesis, gene expression, immunization, antibody purification, identification and antibody labeling services are provided to support your research.
Mouse Monoclonal Antibody Production:
Production of mouse monoclonal antibodies by our hybridoma platform: Customers can provide small molecules, polypeptides, modified polypeptides, proteins, fusion proteins, modified proteins and antigens for monoclonal antibody production. Meanwhile, we also provide antigen customization services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, peptide modification, peptide and carrier protein cross-linking, small molecule structure analysis and carrier protein conjugation. The research team at Gxene Biotech has extensive experience in developing vitro diagnostic antibodies & antigens, especially in the selection and identification of antibodies (such as in vitro detection of antigen immunogenicity). Your questions about the programme, the process, etc. will be answered professionally and in detail by our scientists.
Meanwhile, our patent adjuvant (FMA01# FastAb Mouse Adjuvant) can cause a strong immune response after 21 days of immunization. In addition, we also provide a faster way- surgical spleen cell immunization to save the investigator's time.
Service Process:
Service Content:
|
Procedure |
Description |
Timeline |
|
Immunization |
* Immunization of 5 mice (BALB/c), 3-4 times.
* Sera collection from 5 mice and ELISA titer determination.
* Immunization report will be delivered to the investigator. |
4-6 weeks |
|
Hybridoma Screening |
* Spleen cells (the highest serum titer) fused with mouse myeloma cells.
* Limiting dilution of fusion cells.
* HAT selection.
* Selection of 1-10 positive clones and subcloning to 96 plates.
* Screening positive subclones by ELISA and expansion of cultivation .
* 1mL supernatant/positive clones delivered to the investigator for testing. |
4-6 weeks |
|
Production of Ascites Fluid (Optional) |
* The hybridoma cells selected by the investigator were used to produce ascites, mice were immunized, Protein A/G purified the ascites and ELISA titer determination.
* Purified antibody is delivered to the customer, <5mg/clone.
* Provide detailed report and residual ascites antibody. |
1-3 weeks |
|
Functional Verification (Optional) |
* ELISA, WB, IF, FLAC, IHC, etc. |
1-3 weeks |
Service Highlights:
--Rich experience in monoclonal antibody development.
--High affinity and specificity—pM grade.
--Suitable for production of antibody with inactivated antigen.
--Suitable for Immunohistochemistry of formaldehyde fixation.
--Applicable to different levels of antibody screening protocols, such as epitope-specific antibody screening.
--By using our FastAb Mouse Adjuvant, the antibody titer will peak after 3rd or 4th injection, decrease the immunization timeline and immunogen dosage.
--One-stop downstream services, including antigen production, antibody development, ELISA kit development, etc.
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