To achieve a certain therapeutic effect in vivo, the affinity of the antibody needs to reach a certain level. In addition, the affinity of an antibody decreased obviously after humanization. Therefore, it is necessary to increase the affinity of humanized antibodies to the pre-humanized level. Affinity maturation is a common strategy to improve the antigen-binding affinity of antibodies. Gxene Biotech has established a mature antibody production platform with advanced antibody engineering technology. Gxene Biotech offers antibody affinity maturation services that can improve the affinity of antibodies to the desired level.
Supported by our well-established antibody phage display library, Gxene Biotech can provide affinity maturation services for multi-species antibodies. Together with our experienced scientists in antibody affinity maturation, Gxene Biotech can make the affinity of monoclonal antibodies reach 10-9M. Better quality antibodies to make your research easier.
Antibody Affinity Maturation:
Affinity maturation is the process by which TFH cell-activated B cells produce antibodies with increased affinity for antigens during an immune response. Successive generations of B cells mutate and present to the antigen, that recognizes the antigen with high affinity will survive, and the low-affinity ones are eliminated. Gxene Biotech uses phage display library technology, and combined with error-prone PCR and site-directed mutagenesis, we were able to mimic these over-frequency cellular mutations. Our technical advantage lies in the ability to mutate rapidly in vitro and construct more than 1010 independent clones of antibody phage display libraries, so that high specificity and affinity antibodies can be obtained in a short time.
Service Process:
Common Affinity Maturation Techniques:
In the process of antibody affinity maturation in vitro, the selection of mutation region is a crucial problem, site-directed mutagenesis or random mutagenesis can be performed on the CDR regions.
--Site-directed mutagenesis is based on 3D modeling to identify critical residues involved in the antibody-antigen interaction. Specific mutations on these residues are performed in order to create an extensive phage display library with low junk variants and a high probability of substantial affinity enhancement.
--Random mutagenesis is inserted by the use of error-prone PCR. This method allows the construction of large libraries containing 10
8 starting from 3 to 10 selected antibodies.
Deliverable:
--Affinity optimized antibodies sequences.
--Purified antibody with high affinity.
--Comprehensive report and data: including SDS-PAGE and affinity analysis, etc.
Service Highlights:
--Extensive experience and strong track record in antibody affinity maturation
--Improve the affinity up to 10
-9M
--A variety of methods to ensure a high success rate
--Strict quality control
--Various antibody library searching strategies are available
--One-stop services, including antibody development, antibody sequencing, antibody labeling, antibody humanization and related services
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