The integration of exogenous plasmid DNA into the chromosome of host cells, followed by long-term expression of the target protein, is called a stable cell line. A high-quality stable cell line plays an essential role in biological research, including biosimilar development, gene function research and recombinant antibody production. However, constructing an effective cell line is a complex and time-consuming task. To simplify your experimental steps, Gxene Biotech is committed to improving the quality of our cell lines so that they can be used directly in your downstream biological research. Target cell lines constructed by Gxene Biotech are tested and trusted. We have successfully constructed a large number of target cell lines for many research clients. Based on advanced technology and dedicated research teams, Gxene Biotech can provide the following services:
CRISPR/Cas9 Knock-out Cell Line Services
IOS Overexpression Stable Cell Line Services
IOS RNAi Stable Cell Line Services
Pinpoint Targeted Integration Cell Line Services
IOS Overexpression Stable Cell Line Construction:
Gxene Biotech can provide gene overexpression stable cell line services based on our patented IOS (Integration Operating System) recombination technology. With this technology, the target gene was integrated into the genomic high-expression sites. These sites are safe (no gene) and also have higher gene expression levels (active expression sites), so that the target gene can be overexpressed and stably inherited for a long time. Moreover, gene integration is not limited in length, and multiple genes can be integrated simultaneously to achieve multi-gene co-expression.
The Principle of IOS Technology:
Step 1. Specific oligonucleotide sequences recognize target genes, open double strands and cause conformational changes.
Step 2. The patented RP protein specifically recognizes and binds to target sites, causing DNA damage, thus initiating DNA damage & repair mechanisms.
Step 3. Dimer RP can pull foreign donor genes to the location of DNA damage, greatly improving the probability of recombination.
Fig 1. IOS gene recombination technology
Service Procedure:
Service Highlights
--Customizable: We customize our offering to meet your specific project needs and provide professional scientific support at every step of your project.
--Short time and high integration efficiency.
--Multiple genes can be integrated simultaneously.
--Stable inheritance, the gene will not be lost in the process of passage.
∷ 
Sweep WeChat yards pay attention to us
