Steps | Specification | Deliverables |
Antigen design and synthesis |
* Clients provide target antigen (polypeptide, protein), purity >90%. * We design and synthesize phosphorylated or non-phosphorylated antigen peptides. (Optional) |
(Antigen production by Gxene Biotech) * MS and HPLC reports (for both phosphopeptide and non-phosphopeptide). * 2-5mg phosphopeptide (>90% purity). * 2-5mg non-phosphopeptide (>90% purity). |
Animal immunization |
* Protocol 1: Immunization of 5 Balb/c mouse (using a unique mouse immunoadjuvant to produce high titer antiserum rapidly in 20 days). Detection of serum titer by ELISA. * Protocol 2: Immunization of 2-3 rabbits (using a unique rabbit immunoadjuvant to produce high titer antiserum rapidly in 28 days). Detection of serum titer by ELISA. (Other animals are available) |
* 1-2 ml of pre-immune serum. * ELISA identification report. |
Production of antibody phage display libraries |
1. Collecting B lymphocytes after immunization. 2. mRNA extraction & cDNA synthesis & phage vector construction & transformation & amplification. 3. Screening of antibody library with phosphorylated and non-phosphorylated antigen. 4. Amplification of positive clones & Construction of shuttle plasmids. 5. Small-scale and large-scale mammalian system expression (293, CHO). Antibody purification and identification. |
* Recombinant expression plasmid. * 2-5 mg of purified recombinant antibody. * ELISA results (both for phosphopeptide and nonphosphopeptide). * Western Blot identification report (Optional). |
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