Active Recombinant Human Methionine Aminopeptidase 2/p67 Pro_武汉瑾萱生物科技有限公司
Active Recombinant Human Methionine Aminopeptidase 2/p67 Pro
货号:GX00334
Swiss Prot:P50579-1
种属:Human
表达宿主:Sf9
表达区间:Ala2-Tyr478
标签:C- 10*His tag
纯度:>90%
内毒素:<0.1EU/ug
价格:
500 元
现货/In Stock
规格:
说明书:
产品详情:
产品名称: Active Recombinant Human Methionine Aminopeptidase 2/p67 Pro
别名: rHuMethionine aminopeptidase 2/METAP2, His; Methionine aminopeptidase 2; MAP 2; MetAP 2; p67; p67eIF2; Peptidase M; METAP2; MAP2
货号: GX00334
Swiss Prot: P50579-1
种属: Human
表达宿主: Sf9
表达区间: Ala2-Tyr478
标签: C- 10*His tag
纯度: >90%
实际分子量: 66 kDa
内毒素: <0.1EU/ug
无载体: Yes
无动物源: Yes
制剂: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述: Recombinant Human Methionine Aminopeptidase 2/p67 Protein is produced by Sf9 expression system. The target Protein is expressed with Sequence (Ala2-Tyr478) of (Accession P50579-1) fused with a C- 10*His tag
活性测试: Measure by its ability to remove methionine from a fluorogenic peptide substrate HMet-Gly-ProAMC. The resulting GPAMC is cleaved by Recombinant Human DPPIV/CD26. The specific activity is >15 pmol/min/µg, as measured using the described conditions.
复溶: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
储存: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。

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