Active Recombinant Mouse IL-12 Protein_武汉瑾萱生物科技有限公司
Active Recombinant Mouse IL-12 Protein
货号:GX00318
Swiss Prot:P43431&P43432
种属:Mouse
表达宿主:293F
表达区间:23- 215&23-335 aa
标签:C-His tag(IL-12a)&No tag(IL-12b)
纯度:>95%
内毒素:<0.1EU/ug
价格:
500 元
现货/In Stock
规格:
说明书:
产品详情:
产品名称: Active Recombinant Mouse IL-12 Protein
别名: IL-12; Interleukin 12; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 su
货号: GX00318
Swiss Prot: P43431&P43432
种属: Mouse
表达宿主: 293F
表达区间: 23- 215&23-335 aa
标签: C-His tag(IL-12a)&No tag(IL-12b)
纯度: >95%
实际分子量: 58- 71 kDa
内毒素: <0.1EU/ug
无载体: Yes
无动物源: Yes
制剂: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述: Recombinant Mouse IL-12 Protein is produced by 293F expression system. The target Protein is expressed with Sequence (23- 215&23-335 aa) of (Accession P43431&P43432) fused with a C-His tag(IL-12a)&No tag(IL-12b)
活性测试: Measured in a cell proliferation assay using PHAactivated mouse splenocytes. The ED50 forthis effect is 0.01-0.1 ng/mL.
复溶: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
储存: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。

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