| 货号: | GX00097 |
| Swiss Prot: | P04196 |
| 种属: | Human |
| 表达宿主: | 293F |
| 表达区间: | 19- 525 aa |
| 标签: | C- 10*His tag |
| 纯度: | >90% |
| 内毒素: | <0.1EU/ug |
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500 元
现货/In Stock
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| 产品名称: | Active Recombinant Human HRG Protein |
| 别名: | Pro-neuregulin-1; Neuregulin-1 beta 1; NRG1-beta 1; HRG1-beta 1; EGF; NRG1; GGF; HGL; HRGA; NDF; SMDF; Heregulin β-1; Heregulin; HRG |
| 货号: | GX00097 |
| Swiss Prot: | P04196 |
| 种属: | Human |
| 表达宿主: | 293F |
| 表达区间: | 19- 525 aa |
| 标签: | C- 10*His tag |
| 纯度: | >90% |
| 实际分子量: | 80-85 kDa |
| 内毒素: | <0.1EU/ug |
| 无载体: | Yes |
| 无动物源: | Yes |
| 制剂: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述: | Recombinant Human HRG Protein is produced by 293F expression system. The target Protein is expressed with Sequence (19- 525 aa) of (Accession P04196) fused with a C- 10*His tag |
| 活性测试: | Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. When 1 x 10^5 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent mannerin the presence of Concanvalin A. The ED50 forthis effect is 0.4-2 μg/mL. |
| 复溶: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。 |
| 储存: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。 |
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