活性重组蛋白 Recombinant Chicken Caronte Protein　重组蛋白

研究方向

鸡Caronte是哺乳动物Cerberus的直系同源物，是Cerberus/DAN分泌糖蛋白家族的成员。至少有5个Dan Domain（DAND）家族成员包括DAN（DAND1）、Gremlin/Drm（DAND2）、PRDC（与Dan和Cerberus相关的蛋白质；DAND3）、Cerberus（DAND4）和Coco（DAND5）。Caronte在左侧旁轴中胚层中表达，是鸡胚胎发育过程中负责将左侧位置信息从节点传递到侧板中胚层（LPM）外围的分子。Caronte作为BMP拮抗剂发挥作用，通过干扰bilaterally-producedBMPs的抑制活性来诱导LPM中的Nodal和下游基因。

此外，Caronte对BMPs的拮抗作用诱导Lefty基因在中线的表达。Caronte在免疫沉淀实验中结合BMP-4和BMP-7。重组鸡Caronte制剂已被证明在功能性ELISA中结合BMP-4并在爪蟾胚胎中诱导心脏逆转。

活性测试：

Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos. 80 ng per embryo induces 25-50% heartreversals. Measured by its binding ability in a functional ELISA. Immobilized rchCaronte/Fc Chimera at 5µg/mL (100 µL/well) can bind hBMP-4 with a linearrange of 0.8-100 ng/mL.

Swiss Prot:

Q9PUK2

种属:

Chicken

表达宿主:

293F

表达区间:

(Asp16-Asn272) and (Glu18-Asn272)

实际分子量:

35-45 kDa

标签:

C-His

纯度：

>90% by SDS-PAGE.

内毒素：

＜0.1EU/ug of the protein by LAL method.

制剂：

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述：

Recombinant Chicken Caronte Protein is produced by 293F expression system. The target protein is expressed with sequence ((Asp16-Asn272) and (Glu18-Asn272)) of Chicken Caronte (Accession Q9PUK2) fused with a C-His.

储存：

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶：

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。