活性重组蛋白 Recombinant Human SIGLEC-5 / SIGLEC5 / CD170 / CD33L2 / OBBP2 Protein　重组蛋白

研究方向

Siglec-5 蛋白是一种假定的粘附分子，与唾液酸依赖性细胞结合有关，对 α-2,3-连接和 α-2,6-连接唾液酸显示出相同的亲和力。值得注意的是，Siglec-5 的唾液酸识别位点可能通过与同一细胞表面上的唾液酸的顺式相互作用而受到掩蔽。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells.The ED50 for this effect is 1‑4 µg/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

47.19 kDa

Observed MW

50-60 kDa

标签

C-His

内毒素

<0.1EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Siglec-5/CD170 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys18-Val437) of human Siglec-5/CD170 (Accession #NP_003821.1) fused with a His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding , followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized . They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-5 cDNA encodes a 551 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasma tail . Siglec-5 exists as a disulfide-linked homodimer on the cell surface and is expressed on monocytes, neutrophils and B cells . It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid .

基因ID

8778

Swiss Prot

O15389

别名

SIGLEC5;CD170;CD33L2;OB-BP2;OBBP2;SIGLEC-5