研究方向
Nectin-1 蛋白通过形成亲同性或异嗜性反式二聚体促进细胞与细胞的接触。已知它与 Nectin-3 和 Nectin-4 发生异嗜性相互作用。此外，这种蛋白质还具有促进神经突生长的活性。在微生物感染的情况下，Nectin-1 充当单纯疱疹病毒 1 (HHV-1)、单纯疱疹病毒 2 (HHV-2) 和伪狂犬病病毒 (PRV) 的受体。
生物活性
Immobilized Recombinant Human Nectin-3 at 2 μg/mL (100 μL/well) can bind Recombinant human Nectin-1 with a linear range of 30-122 ng/mL.
种属
Human
表达宿主
HEK293 cells
Calculated MW
63.84 kDa
Observed MW
80-100 kDa
标签
C-hFc&His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human Nectin-1/PVRL1/CD111 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Thr334) of human Nectin-1/PVRL1/CD111 (Accession #NP_002846.3) fused with an Fc, 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息