活性重组蛋白 Recombinant Human IFN-lambda 1 / IL-29 / IFNL1 Protein　重组蛋白

研究方向

IFN-lambda 1 (IL-29) 是 III 型干扰素家族的成员。IFN-lambda 1 主要由成熟的树突状细胞和巨噬细胞产生。成熟的树突细胞、巨噬细胞、肥大细胞和肺泡细胞表达高水平的 IFN-lambda 1。
IFN-lambda 1 通过包含 IFNλ 受体 1 的异二聚体受体复合物发出信号（ IFNLR1) 和 IL-10 受体亚基-β (IL-10RB)。当与受体复合物结合时，Jak1 和 Tyk2 会被激活，并导致 IFN-λR1（胞内结构域，Tyr406 和 Tyr343，Tyr517）的酪氨酸磷酸化，并激活 STAT1 和 STAT2。激活的 STAT1 和 STAT2 招募 IRF-9 形成三聚体转录因子复合物 (ISGF3)，介导抗病毒状态。
IFN-lambda 1 调节感染和自身免疫性疾病中的免疫力。

生物活性

Measured in antiviral assays using HepG2 cells infected with vesicular stomatitis virus.
The ED50 for this effect is typically 10-50 ng/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

20.86 kDa

Observed MW

30-37 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

<0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IFN-lambda 1/IL-29 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gly20-Thr200) of human IL-29 (Accession #NP_742152.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

This protein is also known as cytokine Zcyto21, Interferon lambda-1, IFN-lambda-1, and IFNL1, is a secreted protein which belongs to the IL-28 / IL-29 family. IL-29 is a cytokine with immunomodulatory activity. IL-29 is highly similar in amino acid sequence to the IL-28. IL-28 and IL-29 are induced by viral infection and showed antiviral activity. IL-28 and IL-29 interacted with a heterodimeric class II cytokine receptor that consisted of IL-1 receptor beta (IL-1Rbeta) and an orphan class II receptor chain, designated IL-28Ralpha. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL-29 may play a role in antiviral immunity. IL-29 up-regulates MHC class I antigen expression. It is a Ligand for the heterodimeric class II cytokine receptor composed of IL1RB and IL28RA. The ligand / receptor complex seems to signal through the Jak-STAT pathway.

基因ID

282618

Swiss Prot

Q8IU54

别名

IFNL1;IL-29;IL29