活性重组蛋白 Recombinant Human IL24 / C49A / FISP / IL10B Protein　重组蛋白

研究方向

白细胞介素24（IL-24）属于细胞因子IL10家族，被鉴定为黑色素瘤细胞终末分化过程中诱导的基因。该基因编码的蛋白质表现出选择性诱导多种癌细胞凋亡的显着能力。IL-24 的过度表达会导致多个 GADD 家族基因的表达升高，这与诱导细胞凋亡有关。在黑色素瘤细胞中，IL-24 诱导丝裂原激活蛋白激酶 14 (MAPK7/P38) 和热休克 27kDa 蛋白 1 (HSPB2/HSP27) 磷酸化，但在正常永生黑色素细胞中未观察到这种效应。已报道了编码不同亚型的多种选择性剪接转录物变体，强调了 IL-24 复杂的调控环境。IL-24 在淋巴结 (RPKM 12.3)、脾 (RPKM 9.6) 和各种其他组织中偏向表达，在协调细胞凋亡和免疫反应中发挥着至关重要的作用。

生物活性

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL20 Rα and human IL20 Rβ. The EC50 for this effect is typically 0.05- 0.25 ng/mL.

种属

Human

表达宿主

HEK293 cells

Observed MW

35kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

<0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

表达区间

Gln 53-Leu 207

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL‑24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN‑ gamma, IL‑1 beta, IL‑12 and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL‑24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL‑20 R alpha and IL-20 R beta, which is shared with IL-19 and IL‑20. The second complex is a combination of IL‑22 R and IL-20 R beta, which is shared with IL-20.

Swiss Prot

Q13007

别名

C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein