活性重组蛋白 Recombinant Human IL-1F1 / IL1A / IL1F1 Protein　重组蛋白

研究方向

IL-18R α 在 CD4+ 和 CD8+ T 细胞上表达，但在幼稚 T 细胞上不表达。
人IL-18Rα蛋白的氨基酸序列与小鼠IL-18Rα蛋白的同源性较低。
IL-18Rα是IL-18的受体。IL-18 分泌到细胞外，并逐步与免疫细胞质膜上的 IL-18 受体 α (Rα) 以及 IL-18 受体 β (Rβ) 结合。IL-18/IL-18Rα/IL-18Rβ 三元复合物形成并置 IL-18Rα 和 IL-18Rβ 的细胞内 Toll-Interleukin-1 受体结构域。然后，接头分子骨髓分化因子 88 (MyD88) 可能在 TRAM 的帮助下被招募。MyD88 进一步与 IL-1 受体相关激酶 (IRAK) 4 和 IRAK1/2 相互作用，形成称为 Myddosome 的大分子组装体，随后通过 TRAF6 激活 IKK。最后，信号激活 NF-κB 和丝裂原激活蛋白激酶通路7，从而上调各种炎症细胞因子的表达。
IL-18R α 与 IL-18 结合，IL-18 受体 β 形成信号复合物，诱导多种炎症细胞因子的表达。IL-18R α 抑制 rhIL-2 和 rhIL-18 联合刺激的 IFN-γ 产生。

生物活性

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 1‑10 ng/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

18.89 kDa

Observed MW

21 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-1 alpha Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser113-Ala271) of human IL-1 alpha/IL1A/IL1F1 (Accession #NP_000566.3) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.

基因ID

3552

Swiss Prot

P01583

别名

IL1A;IL-1A;IL1;IL1-ALPHA;IL1F1