活性重组蛋白 Recombinant Human IL-18 R beta / IL-1 R7 / CD218b / ACPL / IL-1R7 Protein　重组蛋白

研究方向

IL-18Rβ（白细胞介素18受体β，又称IL18RAP）是IL-18R复合物的信号转导链，也是IL-1R家族的成员。IL-18R beta 在 T 细胞、NK 细胞和树突状细胞上表达，但在间充质细胞中不常见表达。
人 IL-18R beta 蛋白的氨基酸序列与小鼠IL-18R beta蛋白同源性较低。
IL-18R beta是IL-18的受体。IL-18 通过与 IL-18 α 链 (IL-18Rα) 结合形成信号传导复合物。称为 IL-18 受体 β 链 (IL-18Rβ) 的共受体被募集形成高亲和力复合物[1]。IL-18/IL-18Rα/IL-18Rβ 三元复合物形成并置 IL-18Rα 和 IL-18Rβ 的细胞内 Toll-Interleukin-1 受体结构域。然后，接头分子骨髓分化因子 88 (MyD88) 可能在 TRAM 的帮助下被招募。MyD88 进一步与 IL-1 受体相关激酶 (IRAK) 4 和 IRAK1/2 相互作用，形成称为 Myddosome 的大分子组装体，随后通过 TRAF6 激活 IKK。最后，信号激活 NF-κB 和丝裂原激活蛋白激酶通路7，从而上调各种炎症细胞因子的表达。
IL-18R beta 与 IL-18 结合，IL-18 受体 α 形成信号复合物，诱导多种炎症细胞因子的表达。IL-18R beta 敲低可抑制肿瘤细胞转移。

生物活性

Measured by its ability to inhibit the IL-18- induced response of KG‑1 human acute myelogenous leukemia cells in the presence of Recombinant Human IL‑18 R alpha /IL‑1 R5. The ED50 for this effect is 2‑6 µg/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

39.4 kDa

Observed MW

55-60 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-18 R beta /IL-1 R7/CD218b Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Phe20-Arg356) of human IL-18 R beta /IL-1 R7/CD218b (Accession #O95256) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

This protein is an accessory subunit of the heterodimeric receptor for interleukin 18 (IL18), a proinflammatory cytokine involved in inducing cell-mediated immunity. This protein enhances the IL18-binding activity of the IL18 receptor and plays a role in signaling by IL18. Mutations in this gene are associated with Crohn's disease and inflammatory bowel disease, and susceptibility to celiac disease and leprosy. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

基因ID

8807

Swiss Prot

O95256

别名

IL18RAP;ACPL;CD218b;CDw218b;IL-18R-beta;IL-18RAcP;IL-18Rbeta;IL-1R-7;IL-1R7;IL-1RAcPL;IL18RB