活性重组蛋白 Recombinant Human IL-13RA1 / CD213a1 / CT19 / NR4 Protein　重组蛋白

研究方向

IL13RA1 蛋白在免疫信号传导中发挥着至关重要的作用，与白细胞介素 13 (IL13) 表现出低亲和力结合。当与 IL4RA 结合时，它形成 IL13 的功能性受体，促进细胞对该细胞因子的反应。此外，IL13RA1 与 IL4RA 一起作为白细胞介素 4 (IL4) 信号转导的替代辅助蛋白，尽管它不能取代 IL2RG 增强白细胞介素 2 (IL2) 结合活性的功能。IL-13 受体是一个复杂的组件，包含 IL4R、IL13RA1 和可能的其他组件，表明该信号系统的复杂结构。此外，IL13RA1 与 TRAF3IP1 相互作用，表明它参与多种细胞过程。此外，IL13RA1 与 IL4 相互作用，强调了其在介导白介素信号通路反应中的多方面作用。

生物活性

Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 2.8μg/ml determined by ELISA.

种属

Human

表达宿主

HEK293 cells

Calculated MW

39.89 kDa

Observed MW

55-70 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human IL-13RA1/CD213a1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met 1-Thr 343) of human IL13RA1 (Accession #NP_001551.1) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. IL13RA1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. This subunit together with IL4RA can form a functional receptor for IL13. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. IL13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

基因ID

3597

Swiss Prot

P78552

别名

CD213A1; CT19; IL-13Ra; NR4;IL13RA1;CT19;IL-13Ra;NR4