研究方向

IL-10受体复合物由四个跨膜链的异二聚体组成，其中两个形成IL-10Rα，其中两个形成IL-10Rβ。IL-10R beta 最初称为孤儿受体 CRF2-4，是活性白细胞介素 10 受体复合物的重要辅助亚基。
IL-10R beta 可与 IL 结合-10 通过 JAK-STAT 途径，IL-10 受体复合物的激活导致受体相关蛋白 TYK 和 JAK-1 磷酸化，并最终激活转录因子 STAT3、STAT1 和 STAT5，这些因子调节IL-10 响应基因（包括 c-myc、bcl-2 和 bcl-xL）的表达，启动信号转导。
IL-10R beta 在大多数细胞上表达细胞类型，也是激活五种 II 类细胞因子（IL-10、IL-22、IL-26、IL-28 和 IFNL1）所需的共享细胞表面受体，这些细胞因子在宿主防御、免疫调节中发挥关键作用。其中，IFNLR1/IL10R β二聚体是细胞因子配体 IFNL2 和 IFNL3 的受体，并介导其抗病毒活性。

生物活性

When  Recombinant  Human IL 28 R  alpha /IFN lambda  R1 Fc Chimera is  immobilized at  5.00μg/mL (100μL/well), the  concentration ofconcentration of  Recombinant  Human IL-10 R  beta Fc Chimera  that produces  50% of the  optimal binding  response is found  to be  approximately  0.300-3.00 μg/mL.  

纯度≥ 95 % as determined by SDS-PAGE. 

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

蛋白构建

A DNA sequence encoding the extracellular domainof human IL10Rβ (NP_000619.3) (Met 1-Ser 220) was expressed, fused with a polyhistidine tag at the C-terminus.

Accession#

NP_000619.3

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Met 20

分子量

The recombinant human IL10Rβ comprises 212 amino acids and has a predicted molecular mass of 25 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL10Rβ is approximately 45-48 kDa due to glycosylation.

缓冲液

Lyophilized from sterile PBS, pH 7.4.

Please contact us for any concerns or special requirements.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

别称

CDW210B Protein, Human; CRF2-4 Protein, Human; CRFB4 Protein, Human; D21S58 Protein, Human; D21S66 Protein, Human; IBD25 Protein, Human; IL-10R2 Protein, Human; IL-10RB Protein, Human

IL-10RB/IL-10R beta 

背景信息

Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins is required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn's disease and ulcerative colitis phenotypes. Crohn's disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

全称

interleukin 10 receptor, beta