研究方向
胰岛素样生长因子结合蛋白 (IGFBP) 家族由六种不同的蛋白成员 (IGFBP1-6) 组成，对 IGF-1 和 IGF-2 表现出高度抑制作用。IGFBP-4 是最小的成员，通过与 IGF 结合来调节生物功能。此外，IGFBP-4 介导不依赖于 IGF 的生物活性。IGFBP-4 抑制结直肠癌、乳腺癌和胶质母细胞瘤细胞的生长。IGFBP-4 在严重急性呼吸窘迫综合征的发生中发挥重要的炎症介质作用，与癌症的增殖、迁移和凋亡有关。
生物活性
Measured by its ability to bind Human IGF1. The ED50 for this effect is 1.95-111.32 ng/mL.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human IGFBP-4 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Glu258) of human IGFBP-4 (Accession #NP_001543.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins . IGFBP-4 binds both insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. It circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. There is mounting evidence that IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.
别名
IGFBP4;BP-4;HT29-IGFBP;IBP4;IGFBP-4
