研究方向
胰岛素样生长因子结合蛋白 2 (IGFBP2) 是高亲和力结合蛋白 (IGFBP1-6) 家族的成员，似乎在中枢神经系统的胰岛素样生长因子 (IGF) 调节中发挥控制作用。胰岛素样生长因子结合蛋白 (IGFBP) 通过与分泌的胰岛素样生长因子 (IGF) 结合来调节其作用，并通过以这种结合形式隔离 IGF 来延长 IGF 在细胞外环境和循环中的半衰期。IGFBP 增强或抑制 IGF 对靶细胞的作用；单个 IGFBP 可以抑制或增强 IGF 对骨中成骨细胞的作用。人胰岛素样生长因子结合蛋白 5 (rhIGFBP-5) 是骨骼中储存的一种独特且最丰富的 IGFBP，对羟基磷灰石和细胞外基质蛋白具有高特异性结合亲和力，因此将其及其结合的 IGF 固定在骨骼内。rhIGFBP-5 在体外持续刺激成骨细胞增殖，从而增加成骨细胞的数量。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-2 at 5 μg/mL (100 μL/well) can bind Recombinant Human IGF1 with a linear range of 53-212 ng/mL.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human IGFBP-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu40-Gln328) of human IGFBP-2 (Accession #NP_000588.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP2 in tumorigenesis.
别名
IBP2; IGF-BP53;IGFBP2;IGF-BP53
