研究方向
IFN-γ R1 (CD119) 是 IFN-γ 受体的亚基之一，是 IFN-γ 的受体。IFN-γ R1 组成型表达于几乎所有细胞的表面。
IFN-γ R1 可以与 IFN-γ R2 结合形成功能性受体。与 IFN-γ 结合后，IFNγR1 和 IFNγR2 寡聚并转磷酸化。然后，JAK1 和 JAK2 被磷酸化并激活，STAT1 被招募到受体复合物中。IFNγR1 的磷酸化为 STAT1 创建了一个对接位点，并导致 STAT1 磷酸化。磷酸化的 STAT1 易位到细胞核，在那里调节 IFN 反应基因（例如 CD54）的表达。编码 IFN-gamma R1 的 IFNGR1 基因突变会导致原发性免疫缺陷并导致分枝杆菌感染，例如孟德尔分枝杆菌病易感性 (MSMD)
人类 IFN-gamma R1 由胞外结构域 (E18-G245)、螺旋结构域 (S246-I266) 和胞质结构域 (C267-S489) 组成。IFNAR1的氨基酸序列在不同物种中有所不同。人 IFN-γ R1 与小鼠 IFN-γ R1 的氨基酸序列有 50% 的同一性。IFN-gamma R1 在抗菌、抗病毒和抗肿瘤反应中发挥着关键作用。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97 ng/mL.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human IFN-gamma R1/CD119 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly245) of human IFNGR1/CD119 (Accession #NP_000407.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gammaR1 (IFN gamma R alpha ) and IFN-gammaR2 (IFN-gamma R beta ). IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gammaR2 is required for IFN gamma signaling but does not bind IFN-gamma by itself. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
别名
CD119; IFNGR; IMD27A; IMD27B;IFNGR1; CD119; interferon gamma receptor 1;IFNGR;IMD27A;IMD27B
