研究方向
ICAM-2 (CD102) 与结构相关的 ICAM-1 (CD54) 和 ICAM-3 (CD50) 蛋白一样，是白细胞整联蛋白 ß2 家族的已知配体。这些包括 CD11a/CD18 (LFA-1)、CD11b/CD18 (Mac-1) 和树突状细胞特异性 ICAM 捕获非整合素 (DC-SIGN) 蛋白。ICAM-2 包含两个免疫球蛋白结构域，它们与 ICAM-1 的第一和第二免疫球蛋白结构域同源，没有任何已知的剪接变体。ICAM-2 均匀存在于骨髓细胞悬液中所有 B 淋巴细胞谱系细胞亚群上。血管内皮细胞上的高水平组成型表达以及对炎症刺激的反应性比ICAM-1低也是ICAM-2的特征。重组ICAM-2可以在体外共刺激T细胞。尽管 ICAM-2 介导中性粒细胞迁移，但 ICAM-2 似乎能够介导不依赖于 CD31/PECAM-1 的白细胞迁移。ICAM-2 还可能通过多种机制调节血管生成，包括存活、细胞迁移和 Rac 激活。
生物活性
Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-2 coated plates (12.5 μg/mL, 100μL/well),approximately >40% cells will adhere specifically after 60 minutes at 37 ℃.
种属
Human
表达宿主
HEK293 cells
Calculated MW
48.15 kDa
Observed MW
60-70 kDa
标签
C-hFc&His
纯度
> 97% by SDS-PAGE.
内毒素
Please contact us for more information.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human ICAM-2/CD102 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys25-Gln216) of human ICAM-2 (Accession #NP_000864.2) fused with an Fc, 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-2 coated plates (12.5 μg/mL, 100 μL/well),approximately >40% cells will adhere specifically after 60 minutes at 37 ℃.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息