研究方向
GFRAL 蛋白是 GDF15 的脑干限制性受体，在调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力方面发挥着至关重要的作用。在与其配体 GDF15 结合后，GFRAL 与 RET 相互作用，并通过 MAPK 和 AKT 信号通路激活细胞信号传导。该受体通过其胞外结构域与 GDF15 和 RET 形成复合物，在 GDF15 结合后 RET 参与时特异性介导细胞信号传导。这种复杂的相互作用凸显了 GFRAL、GDF15 和 RET 在调节代谢挑战的生理反应中的连续步骤。
纯度
> 95% as determined by Tris-Bis PAGE.> 95% as determined by HPLC.
内毒素
< 0.1EU per μg by the LAL method.
生物活性
Immobilized Human GFRAL Protein at 0.5 μg/mL (100 μL/well) can bind Human GDF15 with the EC50 of 8.5ng/ml
制剂
Lyophilized from 0.22um filtered solution in 20mM PB, 300mM NaCl (pH 6.5). Normally 5 % trehalose is added as protectant before lyophilization.
描述
Recombinant Human GFR alpha-like/GFRAL Protein is produced by mammalian expression system. The target protein is expressed with sequence (Ser19-Glu351) of Human GFR alpha-like/GFRAL (Accession #) fused with a His tag at the C-terminal. .
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses
别名
GFR alpha-like; GFRAL; GRAL; C6orf144
