研究方向

G-CSFR/CD114 是粒细胞集落刺激因子 (CSF3) 的受体，通过控制中性粒细胞谱系细胞的增殖、分化和存活，在协调粒细胞成熟中发挥着关键作用。这种同二聚体受体与两个 CSF3 分子结合，形成调节重要细胞过程的关键相互作用。除了参与细胞成熟之外，G-CSFR/CD114 还可能有助于细胞表面的粘附或识别事件。此外，它与 CEACAM1 的相互作用充当调节轴，通过招募 PTPN6 下调 CSF3R-STAT3 通路，随后 PTPN6 使 CSF3R 去磷酸化并调节细胞信号传导动力学。

纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 0.1 EU per μg of the protein as determined by the LAL method

生物活性

Measured by its ability to inhibit the GCSF induced proliferation of MNFS-60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this ettect is typically 31.5-126 ng/mL.

蛋白构建

A DNA sequence encoding the extracellular domain (Met 1-Pro 621) of human G-CSF receptor (NP_000751.1) precursor was fused with a polyhistidine tag at the C-terminus.

Accession#

NP_000751.1

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Glu 25

分子量

The mature recombinant human GCSFR consists of 608 amino acids and predicts a molecular mass of 68 kDa by SDS-PAGE under reducing conditions, the apparent molecular mass of rhGCSFR is approximately 92 kDa due to glycosylation.

缓冲液

Lyophilized from sterile PBS, pH 7.4.

Please contact us for any concerns or special requirements.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hardcopy of datasheet.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

别称

CD114 Protein, Human; G-CSF-R Protein, Human; GCSFR Protein, Human; SCN7 Protein, Human

CSF3R/G-CSFR 背景信息

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.