研究方向
FGL1 蛋白作为一种免疫抑制分子，通过作为 LAG3 的主要配体对抗原特异性 T 细胞激活发挥抑制作用。它在独立于 MHC II 类 (MHC-II) 结合介导 LAG3 的 T 细胞抑制功能中发挥着关键作用。除了其免疫调节作用外，FGL1 还由肝细胞分泌，有助于其生长。 FGL1 作为同二聚体存在，通过其纤维蛋白原 C 末端结构域与 LAG3 相互作用，特异性结合 LAG3 的 Ig 样结构域 1 和 2。研究中详细介绍了这种分子相互作用，强调了 FGL1 在调节免疫反应和肝细胞生长中的重要性，强调了其在 T 细胞激活和肝功能调节中发挥关键作用的潜力。
种属
Human
表达宿主
HEK293 cells
Calculated MW
37.22 kDa
Observed MW
36-38 kDa
标签
C-His
纯度
> 92% by SDS-PAGE.
内毒素
Please contact us for more information.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Tris,300mM NaCl,10% Glycerol,pH 8.0Contact us for customized product form or formulation.
描述
Recombinant Human Fibrinogen like 1/FGL1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-IIe312) of Human Fibrinogen like 1/FGL1 (Accession #NP_004458.3) fused with a 6×His tag at the C-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human FGL1 at 5 μg/mL (100 μL/well) can bind recombinant Human LAG3 with a linear range of 0.5-21.6 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human FGL1 at 1 μg/mL (100 μL/well) can bind FGL1 Rabbit mAb with a linear range of 1-27.5 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human FGL1 at 1 μg/mL (100 μL/well) can bind FGL1 Rabbit mAb with a linear range of 1-27.5 ng/mL.
背景信息