活性重组蛋白 Recombinant Human FcERI / Fc-epsilon RI-alpha / FCER1A Protein　重组蛋白

研究方向

Fc epsilon RIA/FCER1A 蛋白作为专为免疫球蛋白 epsilon/IgE 设计的高亲和力受体占据中心地位，充当骨髓细胞中 IgE 效应器功能的关键介质。与 IgE 结合并随后与抗原/过敏原交联后，它会激活诱导骨髓细胞活化和分化的信号传导途径。特别是在肥大细胞、嗜碱性粒细胞和嗜酸性粒细胞上，Fc epsilon RIA 会触发血管活性胺、脂质介质和细胞因子的分泌，从而促进炎症反应、组织重塑和针对微生物的细胞毒性。这种精心策划的反应构成了对过敏原的立即超敏反应的一部分，充当针对蠕虫寄生虫、病原菌和毒液毒性的宿主防御机制。然而，当失调时，这种受体可能会导致有害且危及生命的过敏和过敏反应。在结构上，它以四聚体形式存在，包含一条 α 链、一条 β 链和两条二硫键连接的 γ 链，并通过 CH3 区域与 IGHE 相互作用。

种属

Human

表达宿主

HEK293 cells

Calculated MW

21.87 kDa

Observed MW

38-50 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.01 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human Fc-epsilon RI-alpha/FCER1A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val26-Gln205) of human Fc epsilon RI alpha (Accession #NP_001992) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA.Immobilized FITC anti-human FcεRIα Antibody at 1μg/mL (25 μL/well) can bind Human Fc epsilon RI alpha with a linear range of 0.46-3.15 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

FCER1A is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.

基因ID

2205

Swiss Prot

P12319

别名

FCER1A;FCE1A;FcERI