研究方向
HER2/CD340 蛋白是一种动态蛋白酪氨酸激酶，是多种细胞表面受体复合物中的关键成分，需要辅助受体来实现有效的配体结合。至关重要的是，它作为神经调节蛋白受体复合物的一部分发挥着不可或缺的作用，GP30 被确定为该受体的潜在配体。除了其受体功能外，HER2/CD340 蛋白还复杂地调节外周微管 (MT) 的生长和稳定。激活后，由 ERBB2 激活启动的 MEMO1-RHOA-DIAPH1 信号通路会协调细胞膜上 GSK3B 的磷酸化和随后的抑制。这种策略性抑制可防止 APC 和 CLASP2 的磷酸化，促进它们与细胞膜的结合。值得注意的是，膜结合的 APC 能够将 MACF1 定位到细胞膜上，这是微管捕获和稳定的先决条件。在细胞核内，HER2/CD340 蛋白积极参与转录调控，与 PTGS2/COX-2 启动子中的 5'-TCAAATTC-3' 序列结合以激活转录。此外，它通过 RNA Pol I 参与 rRNA 基因的转录，增强了蛋白质合成，有助于整体细胞生长。HER2/CD340 蛋白的多方面活性强调了其在协调不同细胞过程（从受体信号传导到微管动力学和转录调节）中的核心作用。
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human ErbB-2/HER2/CD340 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr23-Thr652) of human HER2/ErbB2 (Accession #NP_004439.2) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized recombinant human ERBB2 at 10 ng/mL (100 μL/well) can bind Trastuzumab with a linear range of 4-20 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. ErbB2 has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
别名
ERBB2; CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1; MLN19; erb-b2 receptor tyrosine kinase 2;ErbB2;Phospho-ErbB2 (Y1139);CD340;HER-2;HER-2/neu;HER2;MLN 19;NEU;NGL;TKR1;erb-b2 receptor tyrosine kinase 2;HER2/ErbB2;MLN19
