研究方向
Ephrin-B2，也被称为 EFNB2，是 Eph 受体的关键细胞表面跨膜配体，Eph 受体是受体酪氨酸激酶家族，对于协调神经元、血管和上皮发育过程中的迁移、排斥和粘附至关重要。Ephrin-B2 显示出与相邻细胞上的 Eph 受体混杂结合的倾向，可引发接触依赖性双向信号传导，分为受体下游的正向信号传导和肝配蛋白配体下游的反向信号传导。其结合亲和力延伸至受体酪氨酸激酶，包括 EPHA4、EPHA3 和 EPHB4，后者在心脏形态发生和血管生成中形成重要的伙伴关系，控制细胞粘附和迁移。在 EPHB4 介导的前向信号传导中，Ephrin-B2 调节细胞排斥和与 EFNB2 表达细胞的分离，可能影响纵向突出轴突的方向。值得注意的是，Ephrin-B2 在微生物感染过程中作为亨德拉病毒和尼帕病毒的受体发挥着独特的作用，为其多方面的功能增添了有趣的一面。
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human Ephrin-B2/EFNB2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile28-Ala229) of human Ephrin-B2/EFNB2 (Accession #NP_004084.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human EFNB2 at 0.5 μg/mL (100 μL/well) can bind Human EPHB4 with a linear range of 6-400 pg/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
This protein is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
别名
EFNB2;EPLG5;HTKL;Htk-L;LERK5;ephrin-B2; HTKL; Htk-L; LERK5
