研究方向
CD98 蛋白作为伴侣，有助于功能性转运蛋白异二聚体的生物发生和运输至质膜。它与各种 SLC7 家族转运蛋白形成异二聚体，包括 SLC7A5、SLC7A6、SLC7A7、SLC7A8、SLC7A10 和 SLC7A11，充当氨基酸反向转运蛋白，其底物特异性由 SLC7A 亚基决定。例如，异二聚体 SLC3A2/SLC7A11 通过介导细胞外阴离子 L-胱氨酸和细胞内 L-谷氨酸的交换而发挥逆向转运蛋白的作用。此外，SLC3A2/SLC7A5 与 LAPTM4B 结合时，会被募集至溶酶体，促进亮氨酸摄取和 mTORC1 激活。CD98 对于各种 SLC7 转运蛋白的质膜定位、稳定性和转运活性至关重要。此外，它在调节整合素相关信号传导方面发挥着关键作用，有助于细胞扩散、迁移和肿瘤进展。在丙型肝炎病毒 (HCV) 感染的情况下，SLC3A2 和 SLC7A5/LAT1 复合物促进病毒进入宿主细胞并增加 L-亮氨酸摄取，从而增强 mTORC1 信号激活并促进 HCV 介导的发病机制。
表达宿主
Baculovirus-Infected Sf9 Cells
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human SLC3A2/CD98 Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Arg206-Ala630) of human SLC3A2 (Accession #NP_002385.3).
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA.Immobilized Human SLC3A2 Protein at 5 μg/mL (100 μL/well) can bind ITGB1 with a linear range of 0.0195-1.216 μg/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
This protein is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.
别名
4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE;SLC3A2;4F2HC;4T2HC;CD98;CD98HC;MDU1;NACAE
