神经营养蛋白-3 (NT-3) 是神经营养蛋白家族的一种营养因子，广泛表达于神经系统中。
NT-3 的生理作用由两种膜受体的激活介导：低亲和力的膜受体受体p75和高亲和力受体Trk。NT-3 激活 TrkC 受体，并与 TrkB 和 TrkA 结合，亲和力较小。
NT-3 减少细胞损伤，改善不同病变或神经变性模型中的神经元再生，参与突触重组、突触形成和神经元再生。可塑性。
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.Contact us for customized product form or formulation.
描述
Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Tyr139-Thr257) of human NT-3/Neurotrophin-3 (Accession #P20783).
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptideand a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequencesof mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survivalof specific neuronal subpopulations in both the central as well as the peripheral nervous systems.
别名
NTF3;HDNF;NGF-2;NGF2;NT-3;NT3
