研究方向

据推测 ADAM8 蛋白参与白细胞外渗过程，这意味着其在促进白细胞从血管迁移到周围组织中的潜在作用。这表明 ADAM8 可能通过调节白细胞穿过内皮屏障的运动来促进免疫反应，这是炎症和免疫监视过程中的一个重要步骤。

种属

Human

表达宿主

HEK293 cells

Calculated MW

55.44 kDa

Observed MW

60-70 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 50mM Tris,10mM CaCl2,150mM NaCl, pH 7.5.Contact us for customized product form or formulation.

描述

Recombinant Human ADAM8/CD156a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Pro497) of human ADAM8/CD156a (Accession #NP_001100.3) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.

After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；

复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2.The specific activity is >2 pmol/min/μg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

基因ID

101

Swiss Prot

P78325

别名

ADAM8;CD156;CD156a;MS2