活性重组蛋白 Recombinant Human Tau-F/MAPT/TAU Protein　重组蛋白

种属

Human

表达宿主

HEK293 cells

Calculated MW

46.69 kDa

Observed MW

65-80 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

<0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human Microtubule-associated protein tau/MAPT Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Leu441) of human Tau-F/MAPT-F (Accession #NP_005901.2) fused with a 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

研究方向

Microtubule-associated protein tau 是一种微管相关蛋白，在中枢神经系统 (CNS) 的神经元中大量存在。MAPT 促进微管组装和稳定性，并可能参与神经元极性的建立和维持。MAPT 可 C 端结合轴突微管，N 端结合神经质膜成分，作为两者之间的连接蛋白起作用。MAPT 缺陷时会引起神经性疾病如阿尔茨海默病和帕金森病。MAPT 的过表达与前列腺癌的不良预后相关。Tau 蛋白 S262/S356 位点可被 AMPK 相关激酶磷酸化。

背景信息

MAPT (microtubule-associated protein tau) can produce tau proteins. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons of the central nervous system and are less common elsewhere, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer"s disease. Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau"s main functions is to modulate the stability of axonal microtubules. Other nervous system MAPs may perform similar functions, as suggested by tau knockout mice, who did not show abnormalities in brain development - possibly because of compensation in tau deficiency by other MAPs.Tau-F is known as "2N4R," "Isoform Tau-F," "Tau-4" or "Tau 441", which consisting of 441 amino acid. Tau-F is a potential therapeutic target for pathogenesis.

基因ID

4137

Swiss Prot

P10636-8

别名

DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU;MAPT;FTDP-17;MAPTL;MSTD;MTBT1;MTBT2;PPND;PPP1R103;TAU;Tau; DDPAC; microtubule-associated protein tau