活性重组蛋白 Recombinant Human Lumican/LUM Protein
活性重组蛋白 Recombinant Human Lumican/LUM Protein
种 属:Human
商品编号:GX004805
商品库存:100支 浏览:515 次
会员价格:¥980.00元
定购数量:      
详细介绍:    


种属
Human
表达宿主
HEK293 cells
Calculated MW
37.50 kDa
Observed MW
45-60 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human Lumican/LUM Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Asn338) of human Lumican/LUM (Accession #NP_002336.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

研究方向

Lumican,也称为LUM蛋白,是一种具有与层粘连蛋白结合能力的蛋白质。层粘连蛋白是细胞外基质的关键成分,细胞外基质是一个复杂的分子网络,为组织提供结构支持。lumican 与层粘连蛋白的结合表明其参与细胞相互作用和组织组织的调节。然而,需要进一步的研究来充分了解lumican与层粘连蛋白相互作用的具体机制和功能含义,以及它在各种生理和病理过程中的潜在作用。

背景信息

Lumican is a member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily .which is a major component of the cornea, dermal, and muscle connective tissues. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Lumican’s over-expression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers

基因ID
Swiss Prot
别名
LDC; SLRR2D;LUM;SLRR2D;lumican