描述
LIF protein induces terminal differentiation in leukemic cells, promoting hematopoietic and neuronal cell differentiation, and stimulating acute-phase protein synthesis in hepatocytes. Its diverse activities highlight its role in influencing cellular differentiation across various cell types and physiological contexts. LIF Protein, Human (HEK293) is the recombinant human-derived LIF protein, expressed by HEK293 , with tag free.


研究背景
LIF 蛋白具有诱导白血病细胞终末分化的能力。其多种活性包括诱导正常细胞和髓性白血病细胞的造血分化、促进神经元细胞分化以及刺激肝细胞中的急性期蛋白质合成。这些多方面的活动强调了 LIF 在影响各种细胞类型和生理环境的细胞分化中的作用。


生物活性
1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells and the ED50 is typically 0.2-0.8 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 this effect is ≤0.004637 ng/mL, corresponding to a specific activity is ≥2.157×108 units/mg.
3.Immobilized Human LIF at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of ≤0.12 μg/mL determined by ELISA.




Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.004534 ng/mL, corresponding to a specific activity is 2.206×108 units/mg.
种属
Human


表达系统
HEK293


标签
Tag Free


蛋白编号
P15018-1 (S23-F202)


基因 ID
3976  [NCBI]


蛋白结构
N-term
LIF (S23-F202)
Accession # P15018-1
C-term
同用名
LIF; Leukemia inhibitory factor; HILDA; D factor; MLPLI
氨基酸序列
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF


分子量
Approximately 24-55 kDa due to the glycosylation


纯度
Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.


性状
无菌粉末


组分
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Tris, 150 mM NaCl, pH 7.4.


内毒素含量
<1 EU/μg, determined by LAL method.


复溶方法
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).


保存条件 & 期限
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.


运输条件
Room temperature in continental US; may vary elsewhere.