描述
IFN-alpha 1 (IFNA1), belongs to type I interferon family, is produced by macrophages with antiviral activities[1]. IFN-alpha 1 involves in the activation of JAK1 and TYK2 pathway[4], exerts function by inhibiting viral replication as well as modulating immune response[3]. IFN-alpha 1/IFNA1 Protein, Human (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag.
研究背景
IFN-α 1（IFNA1；IFN-α1）属于 α/β 干扰素家族，由巨噬细胞产生，具有抗病毒活性[1]。干扰素（IFN）最初被认为是一种在体外“干扰”病毒复制的物质。IFN-α/β及相关分子被归类为I型干扰素，其他两类分别为II型干扰素（IFN-γ）和III型干扰素（IFN-λ）[2]。
IFN 与三种类型特异性受体之一结合，从而激活 JAK1 和 TYK2[3]。这种信号转导导致 STAT1 和 STAT2 磷酸化，并最终与 IFN 调节因子 9 (IRF9) 相关并形成 IFN 刺激的基因因子 3 (ISGF3) 复合物。因此，ISGF3 复合物诱导 IFN 刺激基因 (ISG) 的转录，随后对先天性和适应性免疫反应产生免疫调节作用[4]。
I 型 IFN 与免疫系统的相互作用系统通过其对树突状细胞 (DC) 的影响，对于产生持久的抗肿瘤反应非常重要[5]。IFN已广泛用于动物疾病模型，人IFNA1蛋白氨基酸序列与小鼠有很大差异（62.96%）。
体外研究
(In Vitro)
IFN-alpha induces highly active dendritic cells (DC) rapidly generated from blood monocytes in vitro, suitable for therapeutic vaccination of cancer research[5].
生物活性
Measured by its ability to inhibit the proliferation of TF-1 human erythroleukemic cells. The ED50 this effect is 0.327 ng/mL, corresponding to a specific activity is 3.058×106 units/mg.
种属
Human
表达系统
HEK293
标签
C-6*His
蛋白编号
P01562 (C24-E189)
基因 ID
3439 [NCBI]
蛋白结构
N-term
IFNA1 (C24-E189)
Accession # P01562
6*His
C-term
中文名
重组人 IFN-alpha 1/IFNA1 蛋白 (HEK293, His)
同用名
rHuInterferon alpha-1, His; Interferon alpha-1/13; IFN-alpha-1/13; Interferon alpha-D; LeIF D; IFNA1; IFNA13
氨基酸序列
CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE
分子量
17-20 kDa
纯度
Greater than 95% as determined by reducing SDS-PAGE.
性状
无菌粉末.
组分
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
内毒素含量
<1 EU/μg, determined by LAL method.
复溶方法
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件 & 期限
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件
Room temperature in continental US; may vary elsewhere.
IFN-alpha 1 (IFNA1), belongs to type I interferon family, is produced by macrophages with antiviral activities[1]. IFN-alpha 1 involves in the activation of JAK1 and TYK2 pathway[4], exerts function by inhibiting viral replication as well as modulating immune response[3]. IFN-alpha 1/IFNA1 Protein, Human (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag.
研究背景
IFN-α 1（IFNA1；IFN-α1）属于 α/β 干扰素家族，由巨噬细胞产生，具有抗病毒活性[1]。干扰素（IFN）最初被认为是一种在体外“干扰”病毒复制的物质。IFN-α/β及相关分子被归类为I型干扰素，其他两类分别为II型干扰素（IFN-γ）和III型干扰素（IFN-λ）[2]。
IFN 与三种类型特异性受体之一结合，从而激活 JAK1 和 TYK2[3]。这种信号转导导致 STAT1 和 STAT2 磷酸化，并最终与 IFN 调节因子 9 (IRF9) 相关并形成 IFN 刺激的基因因子 3 (ISGF3) 复合物。因此，ISGF3 复合物诱导 IFN 刺激基因 (ISG) 的转录，随后对先天性和适应性免疫反应产生免疫调节作用[4]。
I 型 IFN 与免疫系统的相互作用系统通过其对树突状细胞 (DC) 的影响，对于产生持久的抗肿瘤反应非常重要[5]。IFN已广泛用于动物疾病模型，人IFNA1蛋白氨基酸序列与小鼠有很大差异（62.96%）。
体外研究
(In Vitro)
IFN-alpha induces highly active dendritic cells (DC) rapidly generated from blood monocytes in vitro, suitable for therapeutic vaccination of cancer research[5].
生物活性
Measured by its ability to inhibit the proliferation of TF-1 human erythroleukemic cells. The ED50 this effect is 0.327 ng/mL, corresponding to a specific activity is 3.058×106 units/mg.
种属
Human
表达系统
HEK293
标签
C-6*His
蛋白编号
P01562 (C24-E189)
基因 ID
3439 [NCBI]
蛋白结构
N-term
IFNA1 (C24-E189)
Accession # P01562
6*His
C-term
中文名
重组人 IFN-alpha 1/IFNA1 蛋白 (HEK293, His)
同用名
rHuInterferon alpha-1, His; Interferon alpha-1/13; IFN-alpha-1/13; Interferon alpha-D; LeIF D; IFNA1; IFNA13
氨基酸序列
CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE
分子量
17-20 kDa
纯度
Greater than 95% as determined by reducing SDS-PAGE.
性状
无菌粉末.
组分
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
内毒素含量
<1 EU/μg, determined by LAL method.
复溶方法
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件 & 期限
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件
Room temperature in continental US; may vary elsewhere.