纯度
> 95 % as determined by SDS-PAGE
内毒素
< 0.1 EU per μg of the protein as determined by the LAL method
生物活性
Measured by the production of urea during the hydrolysis of arginine.
The specific activity is >35,000 pmoles/min/μg.
The specific activity is >35,000 pmoles/min/μg.
蛋白构建
A DNA sequence encoding the human ARG1 isoform 1 (P05089-1) (Met 1-Lys 322) was fused with a polyhistidine tag at the C-terminus.
Accession#
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Met 1
分子量
The secreted recombinant human ARG1 consists of 333 amino acids and has a calculated molecular mass of 36.2 kDa. The apparent molecular mass of the protein is approximately 40 kDa in SDS-PAGE under reducing conditions.
缓冲液
Supplied as sterile 20mM Tris, 150mM NaCl, 20% Glycerol, 1mM DTT, pH 7.4
Please contact us for any concerns or special requirements.
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hardcopy of datasheet.
运输方式
Liquid. It is shipped out with blue ice.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
精氨酸酶-1 (ARG1) 是尿素循环的核心组成部分，对于 L-精氨酸转化为尿素和 L-鸟氨酸至关重要，后者是胶原蛋白合成和促进细胞增殖的生物能途径中至关重要的代谢物的前体。尿素循环主要在肝脏中活跃，其次在肾脏中活跃，在维持 L-精氨酸稳态方面发挥着关键作用，特别是在一氧化氮合酶 (NOS) 和精氨酸酶对细胞内精氨酸存在竞争关系的组织中。。除了其代谢功能之外，ARG1 还作为先天性和适应性免疫反应的关键调节因子，参与多形核粒细胞 (PMN) 的抗菌效应途径。PMN 细胞死亡后，ARG1 被释放，耗尽微环境中的精氨酸，抑制 T 细胞和自然杀伤 (NK) 细胞的增殖以及细胞因子的分泌。值得注意的是，在第 2 组先天淋巴细胞 (ILC2) 中，ARG1 会导致肺部急性 2 型炎症，影响 ILC2 的最佳增殖。此外，ARG1 在替代激活巨噬细胞或 M2 巨噬细胞的免疫反应中发挥多方面的作用，影响伤口愈合、组织再生、防御多细胞病原体和免疫抑制等过程，其结果因器官而异。在肿瘤浸润树突状细胞 (DC) 和骨髓源性抑制细胞 (MDSC) 中，ARG1 有助于抑制 T 细胞介导的抗肿瘤免疫，凸显其多样化且依赖于环境的免疫调节功能。
Arginase 1/ARG1 背景信息
Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.
全称
arginase 1
参考文献
- Durante W, et al. (2007) Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 34(9): 906-11.
- Waddington SN. (2002) Arginase in glomerulonephritis. Kidney Int. 61(3): 876-81.
- Morris SM. (2002). Regulation of enzymes of the urea cycle and arginine metabolism. Annual review of nutrition. 22 (1): 87-105.