The CD59 protein is a potent inhibitor of the complement membrane attack complex (MAC) and binds to assembled C8 and/or C9 complement, preventing the incorporation of multiple C9 copies that are critical for permeability pore formation. Its species-specific inhibitory effects extend to T cell activation, complexing with protein tyrosine kinases for signal transduction. CD59 Protein, Human (HEK293, His) is the recombinant human-derived CD59 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD59 Protein, Human (HEK293, His) is 77 a.a., with molecular weight of 15-20 kDa.

CD59 蛋白是补体膜攻击复合物 (MAC) 作用的有效抑制剂。它通过与组装 MAC 的 C8 和/或 C9 补体结合来发挥作用，从而阻止渗透孔形成所需的 C9 多个拷贝的掺入。值得注意的是，该抑制剂表现出物种特异性。此外，CD59 与蛋白酪氨酸激酶复合参与信号转导的 T 细胞激活。值得注意的是，虽然尿液中的可溶性 CD59 保留了其特异性补体结合活性，但其抑制 MAC 在细胞膜上组装的能力显着降低。

Human

HEK293

C-6*His

P13987 (L26-N102)

966  [NCBI]

CD59 glycoprotein; HRF-20; MAC-IP; MACIF; MIRL; CD59; MIC11; MIN1; MSK21

LQCYNCPNPTADCKTAVNCSSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLEN

15-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.