描述
Recombinant Human LMIR2/CD300c Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly 21 - Arg 183 ) of human CD300C (Accession #NP_006669.1) fused with a 6×His tag at the C-terminus.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
研究方向
CD300c 蛋白，也称为 CMRF35 抗原，已通过与单克隆抗体的反应来鉴定。Jackson 等人的一项研究记录了这种抗原存在于各种免疫细胞上，包括单核细胞、中性粒细胞以及某些 T 和 B 淋巴细胞。1992 年。该蛋白在多种组织中广泛表达，尤其在肺、阑尾和其他 19 种组织中明显存在。
背景信息
The recombinant human CD300C consists of 166 amino acids with a molecular weight of 18.4 kDa. The apparent molecular mass of recombinant human CD300C is about 40-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. CD300C is present on the surface of natural killer cells, granulocytes, most myeloid cells, dendritic cells, and a subpopulation of T and B lymphocytes. Mouse CD300C has the characteristics of an activatory molecule capable of inducing cellular activation and effector function in most cells and macrophages. The ligand for CD300C is presently unknown.
别名
CD300C;CLM-6;CMRF-35;CMRF-35A;CMRF35;CMRF35-A1;CMRF35A;CMRF35A1;IGSF16;LIR
