种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human BTN1A1/Butyrophilin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 27 - Arg 242 ) of human BTN1A1/Butyrophilin (Accession #NP_001723.2) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat cells. The ED50 for this effect is 3.35-13.4 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
研究方向
BTN1A1 蛋白被认为在乳脂滴的分泌中发挥作用，表明它可能参与与哺乳相关的复杂过程。此外，如相似性分析所示，它可能充当特定的膜相关受体，促进细胞质液滴与顶端质膜的结合。此外，BTN1A1对抗CD3抗体激活的CD4和CD8 T细胞增殖具有抑制作用，影响T细胞代谢，调节IL2和IFNG的分泌，凸显了其在免疫调节中的潜在作用。值得注意的是，BTN1A1 似乎还与黄嘌呤脱氢酶/氧化酶相关，这引发了关于其多样化细胞功能以及在各种生物途径中的潜在影响的进一步问题。
背景信息