种属
Human
表达宿主
E. coli
描述
Recombinant Human Apolipoprotein A-I/APOA1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Asp25-Gln267) of human Apolipoprotein A-I (Accession #NP_000030.1).
标签
No tag
纯度
> 95% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
研究方向
载脂蛋白 AI 通过促进胆固醇从组织流出并充当卵磷脂胆固醇酰基转移酶 (LCAT) 的辅助因子，在胆固醇逆向转运中发挥着至关重要的作用。它还作为 SPAP 复合体的一部分发挥作用，激活精子活力。载脂蛋白 AI 形成同二聚体并与 NAXE 和 CLU 相互作用，同时与免疫球蛋白重链、免疫球蛋白轻链和白蛋白一起成为 SPAP 复合物的组成部分。此外，它还与 NDRG1、SCGB3A2、NAXE 以及 YJEFN3 相互作用。
背景信息
This protein is the major protein component of high density lipoprotein (HDL) in plasma. The preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters.