种属
Human
表达宿主
HEK293 cells
描述
Active Recombinant Human R-spondin-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.
2.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data)
3.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng/mL R-Spondin 1.
2.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data)
3.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng/mL R-Spondin 1.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.