种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse CXCL15/Lungkine Protein is produced by Human cells expression system. The target protein is expressed with sequence (Gln26-Ala167) of mouse CXCL15/Lungkine (Accession #Q9WVL7) fused with a 6×His tag at the C-terminus.
标签
C-6×His
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
背景信息
Mouse C-X-C motif chemokine 15, also known as Cxcl15, is a secreted protein which is member of the ELRmotif-containing CXC chemokines. It expressed at low levels in fetal lung, the expression restricted to the lung,produced by bronchoepithelial cells and is released into the airways. It plays an important role in lung-specificneutrophil trafficking during normal and inflammatory conditions. It also appears chemotactic for neutrophils.