研究方向
重组人脂联素可用于受损动脉并减轻血管炎症反应。据报道，生理浓度的重组人脂联素可抑制肿瘤坏死因子-α(TNF-α) 诱导的内皮粘附分子表达、巨噬细胞向泡沫细胞的转化以及巨噬细胞中 TNF-α 的表达。重组人脂联素可作为治疗糖尿病肌腱病的潜在蛋白质，在体外促进肌腱细胞祖细胞增殖和肌腱分化。
纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU per μg protein as determined by the LAL method.
生物活性
Measured by its ability to induce TIMP-1 secretion by mouse macrophages. The ED50 for this effect is 1-5 µg/mL.
蛋白构建
A DNA sequence encoding the human ADIPOQ (NP_001171271.1) (Met1-Asn244) was expressed with a polyhistidine tag at the C-terminus.
NP No.
表达宿主
HEK293F
种属
Human
预测 N 端
Glu 19
分子量
The recombinant human ADIPOQ consists of 237 amino acids and predicts a molecular mass of 26 kDa.
缓冲液
Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0, 10 % glycerol.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer
Adiponectin Protein, Human, Recombinant (His Tag): 别称
ACDC Protein, Human; ACRP30 Protein, Human; Adiponectin Protein, Human; ADIPQTL1 Protein, Human; ADPN Protein, Human; APM-1 Protein, Human; APM1 Protein, Human; GBP28 Protein, Human
Adiponectin 背景信息
Adiponectin (ADIPOQ), or 30 kDa adipocyte complement-related protein (Acrp30) is a protein secreted by adipose tissue, which acts to reduce insulin resistance and atherogenic damage, but it also exerts actions in other tissues. Adiponectin mediates its actions in the periphery mainly via two receptors, AdipoR1 and AdipoR2. Adiponectin influences gonadotropin release, normal pregnancy, and assisted reproduction outcomes. Adiponectin, a beneficial adipokine, represents a major link between obesity and reproduction. Higher levels of adiponectin are associated with improved menstrual function and better outcomes in assisted reproductive cycles. Unlike other adipocytokines produced by adipose tissue, adiponectin appears to have anti-inflammatory, anti-diabetic, and anti-atherogenic properties. Several clinical studies demonstrate the inverse relationship between plasma adiponectin levels and several inflammatory markers including C-reactive protein. Adiponectin attenuates inflammatory responses to multiple stimuli by modulating signaling pathways in a variety of cell types. The anti-inflammatory properties of adiponectin may be a major component of its beneficial effects on cardiovascular and metabolic disorders including atherosclerosis and insulin resistance. Additionally, it is important factor in chronic liver diseases and chronic kidney diseases. Some cancer cell types express adiponectin receptors. Thus Adiponectin may act on tumour cells directly by binding and activating adiponectin receptors and downstream signalling pathways.
全称
adiponectin, C1Q and collagen domain containing