研究方向
Human LRG1 是由 LRG1 基因编码的 分泌型糖蛋白,属于富含亮氨酸重复序列(LRR)蛋白家族,主要在肝脏合成并广泛分布于体液(如血浆、脑脊液)。其核心功能包括:
生物活性
Measured by its binding ability in a functional ELISA. When human Cytochrome c is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human LRG1 that produces 50% of the optimal binding response is 3-15 ng/mL.
Swiss Prot:
P02750
种属:
Human
表达宿主:
293F
表达区间:
Val36-Gln347
实际分子量:
40- 60 kDa
标签:
C-His
纯度:
>95% by SDS-PAGE.
内毒素:
<0.1EU/ug of the protein by LAL method.
制剂:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述:
Recombinant Human Human LRG1 Protein is produced by 293F expression system. The target protein is expressed with sequence (Val36-Gln347) of Human Human LRG1 (Accession P02750) fused with a C-His.
储存:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of
receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。