研究方向
Human Pentraxin 2/SAP 是由 APCS 基因编码的 五聚体模式识别蛋白,属于短链五聚蛋白家族,主要由肝脏合成并分泌至血液。其核心功能包括:
生物活性
Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RllA/CD32a (R167) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Pentraxin 2/SAP that produces 50% of the optimal binding response is 0.175-1 μg/mL.
Swiss Prot:
P02743
种属:
Human
表达宿主:
293F
表达区间:
His20-Val223
实际分子量:
27- 33 kDa
标签:
C- 10*His tag
纯度:
>95% by SDS-PAGE.
内毒素:
<0.1EU/ug of the protein by LAL method.
制剂:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述:
Recombinant Human Human Pentraxin 2/SAP Protein is produced by 293F expression system. The target protein is expressed with sequence (His20-Val223) of Human Human Pentraxin 2/SAP (Accession P02743) fused with a C- 10*His tag.
储存:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of
receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。