研究方向
Human TGF beta 1是调控细胞增殖、分化及免疫平衡的核心细胞因子，参与发育、修复与疾病进程。
1. 结构与功能
聚焦时空特异性递药系统（如pH响应纳米颗粒）以降低全身毒性。
生物活性
Measured by its ability to inhibit the IL-4- dependent proliferation of HT‑2 mouse T cells. The ED50 forthis effect is 10-70 ng/mL before acid activation.
Swiss Prot:
P01137
种属:
Human
表达宿主:
293F
表达区间:
Leu30-Ser390(C33S)
实际分子量:
13&42&60 kDa
标签:
N- 8*His
纯度：
>95% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Human Human TGF beta 1 Protein is produced by 293F expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of Human Human TGF beta 1 (Accession P01137) fused with a N- 8*His.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
