研究方向
Human Neurofascin 是一种细胞粘附分子，主要表达于神经系统中的轴突和郎飞结（Nodes of Ranvier）。它在神经系统的发育、轴突导向和神经信号传导中发挥重要作用。以下是关于 Human Neurofascin 的详细信息：
1. 基本概述
2. 生物学作用
3. 研究与疾病关联
4. 治疗潜力
5. 最新研究进展
生物活性
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Neurofascin, immobilized at 6 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Swiss Prot:
O94856-3
种属:
Human
表达宿主:
293F
表达区间:
Ile25-Trp1039
实际分子量:
120- 140 kDa
标签:
C-His
纯度：
>95% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Human Human Neurofascin Protein is produced by 293F expression system. The target protein is expressed with sequence (Ile25-Trp1039) of Human Human Neurofascin (Accession O94856-3) fused with a C-His.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
