研究方向
Human GFR alpha 3 (GFRA3) 是一种糖基磷脂酰肌醇（GPI）锚定蛋白，属于 GDNF 家族受体 α（GFRα）亚家族成员。它主要作为胶质细胞源性神经营养因子（GDNF）家族配体的共受体，参与神经元的存活、发育和功能调节。以下是关于 Human GFRA3 的详细信息：
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Artemin at 1 µg/mL can bind Recombinant Human GFR alpha‑3/GDNF R alpha‑3 with an apparent KD < 2 nM.
Swiss Prot:
O60609
种属:
Human
表达宿主:
293F
表达区间:
Asp32-Asn374
实际分子量:
50 kDa
标签:
C-His
纯度：
>95% by SDS-PAGE.
内毒素：
＜0.1EU/ug of the protein by LAL method.
制剂：
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述：
Recombinant Human Human GFR alpha 3/GFRA3 Protein is produced by 293F expression system. The target protein is expressed with sequence (Asp32-Asn374) of Human Human GFR alpha 3/GFRA3 (Accession O60609) fused with a C-His.
储存：
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶：
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
