研究方向
Mouse YM1/Chitinase 3-like 3(CHI3L3)是一种分泌型蛋白,属于几丁质酶3样蛋白家族。它在免疫调节、组织修复和炎症反应中发挥重要作用。以下是关于Mouse YM1/CHI3L3的详细信息:
Mouse YM1/Chitinase 3-like 3(CHI3L3)是一种重要的免疫调节蛋白,在组织修复、炎症反应和免疫应答中发挥关键作用。其在疾病治疗中的潜在应用价值使其成为研究热点。
1. 结构与功能
2. 生物学意义
3. 研究与临床应用
4. 表达与调控
总结
生物活性
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 10^4 cells/well are added to Recombinant Mouse YM1/Chitinase 3- like 3 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hourincubation at 37 °C. The ED50 forthis effect is 0.3-1.2 μg/mL.
Swiss Prot:
O35744
种属:
Mouse
表达宿主:
293F
表达区间:
Met1-Tyr398
实际分子量:
43
kDa
标签:
C-His
纯度:
>95% by SDS-PAGE.
内毒素:
<0.1EU/ug of the protein by LAL method.
制剂:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述:
Recombinant Mouse Mouse YM1/Chitinase 3-like
3 Protein is produced by 293F expression system. The target protein is expressed with sequence (Met1-Tyr398) of Mouse Mouse YM1/Chitinase 3-like
3 (Accession O35744) fused with a C-His.
储存:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。